vitawell-collagen-marine-peptides-reviews The VIVIT peptide is a potent and selective inhibitor that targets the interaction between calcineurin and the Nuclear Factor of Activated T-cells (NFAT). This specific mechanism of action makes it a valuable tool in research for understanding and modulating NFAT signaling pathways, which play crucial roles in various cellular processes, including immune responses and cardiovascular development. Researchers utilize VIVIT peptide to investigate the downstream effects of NFAT activation and to explore potential therapeutic applications in conditions where aberrant NFAT signaling is implicated.
The Nuclear Factor of Activated T-cells (NFAT) is a family of transcription factors that are critical regulators of gene expression in response to extracellular signals, particularly calcium influx. The activation of NFAT typically involves its dephosphorylation by calcineurin, a calcium- and calmodulin-dependent serine/threonine phosphatase. Once dephosphorylated, NFAT proteins translocate to the nucleus, where they bind to specific DNA sequences and regulate the transcription of target genes.
The VIVIT peptide is designed to mimic a specific region of NFAT that binds to calcineurin.NFAT Inhibitor (CAS 249537-73-3) By occupying this binding site on calcineurin, VIVIT effectively blocks the dephosphorylation of NFAT, thereby preventing its activation and subsequent nuclear translocation.Peptide 11R-VIVIT stimulates osteoblastogenesis through ... This targeted inhibition means that VIVIT peptide interferes with the calcineurin-NFAT interaction without directly inhibiting the phosphatase activity of calcineurin itself, preserving its other functions and minimizing off-target effects.NFAT pulls the strings during CD4 + T helper cell effector functions This selectivity is a key advantage in research settings.
The selective inhibition of the calcineurin-NFAT pathway by VIVIT peptide has opened avenues for research in several key areas:
* Immunology and Inflammation: NFAT proteins are central to the activation and function of T-cells and other immune cells作者：J Aramburu·1999·被引用次数：795—TheVIVIT peptide constitutes a highly selective inhibitor of NFAT, which can now be used for direct identification of. NFAT target genes in these various cell.. VIVIT peptide has been employed to study the role of NFAT in immune cell differentiation, cytokine production (such as IL-2, IL-12, and TNF), and inflammatory responses. For instance, studies have shown that VIVIT can inhibit the production of inflammatory mediators, suggesting its potential in modulating inflammatory diseasesStructure-Based Optimization of a Peptidyl Inhibitor against ....
* Cardiovascular Disorders: Aberrant calcineurin-NFAT signaling has been linked to the development of cardiac hypertrophy and other cardiovascular conditionsSpecific NFAT Inhibitor (VIVIT-16). Research using VIVIT peptide aims to elucidate the precise mechanisms by which this pathway contributes to cardiac remodeling and to explore its therapeutic potential in treating heart-related diseases.
* Other Cellular Processes: Beyond immunity and the cardiovascular system, NFAT transcription factors are involved in the regulation of various cellular processes, including cell proliferation, differentiation, and apoptosis. VIVIT peptide serves as a research tool to investigate NFAT's role in these diverse cellular functions across different tissue typesTherapeutic potential of VIVIT, a selective peptide inhibitor ....
To enhance its utility, various forms and modifications of the VIVIT peptide have been developed:
* Cell-Permeable Variants: Standard peptides often struggle to cross cell membranes efficiently. Modified versions, such as 11R-VIVIT, incorporate cell-penetrating sequences (like the poly-arginine tag) to improve intracellular delivery and bioavailability, making them more effective for in vitro and in vivo studiesSelective inhibition of calcineurin-NFAT signaling by ....
* Fusion Proteins: In some research applications, VIVIT has been expressed as a fusion protein, for example, with Green Fluorescent Protein (GFP). This allows for visualization and tracking of the peptide within cells, aiding in the study of its localization and mechanism of action.
* Fluorescently Labeled Peptides: Fluorescent VIVIT peptide has been used as a specific probe to study calcineurin-NFAT binding sites, facilitating the identification of interactions and the development of other inhibitors.
These engineered VIVIT variants offer researchers greater control and flexibility in designing experiments to probe the intricacies of the calcineurin-NFAT signaling pathway.
The VIVIT peptide stands as a crucial research reagent for dissecting the complex calcineurin-NFAT signaling cascade. Its ability to selectively inhibit the interaction between these two key proteins allows for precise investigation into NFAT's diverse roles in immunological, cardiovascular, and other cellular functions. With the development of modified and cell-permeable versions, the VIVIT peptide continues to be an indispensable tool for advancing our understanding of fundamental biological processes and exploring novel therapeutic strategies.
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