peptide-คือ-อะไร Collagen peptide mass fingerprinting (PMF) is a sophisticated analytical technique that leverages mass spectrometry to identify proteins at the sequence level, enabling precise species identification from biological samplesPMF involves breaking proteins down into peptidesusing enzymes like trypsin, then using mass spectrometry to measure the peptides' masses and compare them to .... This method is particularly valuable for analyzing collagen, a ubiquitous structural protein found in connective tissues, which yields a unique "fingerprint" of peptides upon enzymatic digestion作者：K Dierickx·2022·被引用次数：21—Peptide mass fingerprinting of preserved collagenin archaeological fish bones for the identification of flatfish in European waters. Katrien Dierickx .... By comparing the measured masses of these peptides to theoretical predictions, researchers can accurately distinguish between different species, even from degraded or ancient materials.作者：M Buckley·2016·被引用次数：82—Species Identification of Bovine, Ovine and Porcine Type 1 Collagen; Comparing Peptide Mass Fingerprintingand LC-Based Proteomics Methods. by. Mike Buckley. This approach has revolutionized fields ranging from archaeology and paleontology to food authentication and forensic science, offering a powerful tool for understanding biodiversity and provenance.
At its core, collagen peptide mass fingerprinting involves a series of analytical steps designed to generate a reproducible and interpretable datasetPeptide mass fingerprinting of preserved collagen in .... The process begins with the extraction of collagen from a biological samplePeptide mass fingerprinting of preserved collagen in .... This collagen is then subjected to enzymatic digestion, typically using trypsin, which cleaves the protein at specific amino acid residues.This study explores the use ofrapid collagen peptide mass fingerprintingto authenticate flatfish species in the food industry, addressing the issue of ... This enzymatic action breaks down the long collagen chains into smaller peptides.
Following digestion, these peptide mixtures are analyzed using mass spectrometry.Peptide mass fingerprinting of preserved collagen in ... This powerful analytical instrument measures the mass-to-charge ratio of individual ions, effectively determining the precise mass of each peptide fragmentCollagen Fingerprinting: A New Screening Technique for ... - NIH. The resulting data is a spectrum of peptide masses, which serves as the "fingerprint" for the original collagen sample. This mass spectrum can then be compared against databases of theoretical peptide masses derived from known protein sequences. By matching a significant number of observed peptide masses to a specific species' collagen sequence, confident identification can be achieved.Semi-supervised machine learning for automated species ...
The versatility of collagen peptide mass fingerprinting makes it applicable to a wide array of scientific investigations:
Archaeology and Paleontology: One of the most significant applications of collagen peptide mass fingerprinting is in the identification of ancient animal remains. In archaeological contexts, fragmented bones or other organic materials may not be morphologically identifiable to species. PMF allows researchers to identify the species of origin for these remains, providing crucial insights into past diets, trade networks, and environmental conditions.作者：M Buckley·2024·被引用次数：4—Therefore, the application ofcollagen peptide mass fingerprintingto elasmobranchs offers great potential to improve our understanding of their ... This technique is particularly effective for analyzing preserved collagen in archaeological fish bones and other ancient tissues, enabling the distinction between multiple species, such as different types of flatfish.Semi-supervised machine learning for automated species ...
Food Industry and Authentication: Ensuring the authenticity and origin of food products is a growing concern. Collagen peptide mass fingerprinting can be employed to authenticate species in various food items, particularly those derived from animal sources. For instance, it can verify the species of fish used in processed products, preventing mislabeling and fraud. This rapid collagen peptide mass fingerprinting technique is crucial for identifying species in the food industry, addressing issues of adulteration and ensuring consumer confidence.Peptide mass fingerprinting – Knowledge and References
Forensic Science and Wildlife Crime: In forensic investigations, identifying the species of origin for biological evidence, such as bone fragments or hair, can be critical.Quantifying Bone Collagen Fingerprint Variation Between ... Collagen peptide mass fingerprinting offers a reliable method for species identification in such scenarios, aiding in criminal investigations and the fight against illegal wildlife trade.Rapid collagen peptide mass fingerprinting as a tool to ... It can help determine if animal products in illegal trade are derived from endangered speciesSpecies identification by peptide mass fingerprinting (PMF) ....
Zooarchaeology by Mass Spectrometry (ZooMS): Often referred to as ZooMS, collagen peptide mass fingerprinting has become a cornerstone of zooarchaeological research. This high-throughput method allows for the rapid and cost-effective identification of animal remains from archaeological sites, contributing significantly to our understanding of past human-animal interactions and ecosystemsHigh-throughput microCT and ZooMS collagen fingerprinting .... ZooMS utilizes peptide mass fingerprinting to differentiate between taxa based on variations in the amino acid sequences of collagen type I protein.
The widespread adoption of collagen peptide mass fingerprinting stems from several key advantages.Semi-supervised machine learning for automated species ... It is a highly sensitive technique capable of identifying species from even small or degraded samples. The method is also relatively rapid, especially with advancements in high-throughput analysis.作者：K Dierickx·2023·被引用次数：8—Previously, archaeological research has usedpeptide mass fingerprintingto identify fish remains to species by extracting collagen Type I ... Furthermore, it can differentiate between closely related species that might be difficult to distinguish morphologically.
However, certain considerations are important. The quality and preservation of the collagen sample can significantly impact the results.作者：M Buckley·2024·被引用次数：4—Therefore, the application ofcollagen peptide mass fingerprintingto elasmobranchs offers great potential to improve our understanding of their ... Very old or poorly preserved samples may yield fragmented or incomplete peptide mass fingerprints, potentially leading to ambiguous identifications. The availability of comprehensive reference databases is also crucial for accurate species assignment. Additionally, while the core principle is straightforward, the interpretation of complex spectra and the distinction between subtle variations require expertise.作者：M Buckley·2022·被引用次数：18—We explore the potential ofcollagen peptide mass fingerprinting(also known as Zooarchaeology by Mass Spectrometry, or ZooMS) for identifying fish remains Researchers are continuously working to refine the methodology, for example, through sequence-driven species identification of collagen, to enhance accuracy and broaden its applicability.
The field of collagen peptide mass fingerprinting continues to evolve, driven by technological advancements and expanding research applications. The integration of machine learning and artificial intelligence is paving the way for automated species identification, further increasing throughput and efficiencyPMF involves breaking proteins down into peptidesusing enzymes like trypsin, then using mass spectrometry to measure the peptides' masses and compare them to .... Ongoing research also focuses on developing more robust extraction protocols for challenging samples and expanding the scope of analysis beyond collagen type I to other protein types.Collagen Fingerprinting: A New Screening Technique for ... - NIH As these developments unfold, collagen peptide mass fingerprinting will undoubtedly remain an indispensable tool for unraveling biological mysteries and ensuring the integrity of our natural and cultural heritage.
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